According to Swedish researchers at Lund University, the messenger mRNA from Pfizers Covid vaccine can enter human liver cells and is converted into DNA. The. Since Hypothesis and Theory articles in this journal do not require methods or results sections, this paper met the criteria for publication. National Library of Medicine Once the string of amino acids is created in the cell, chemical and physical forces, such as the charges and shapes of the amino acids in the sequence as well as other factors in the cellular environment, cause the newly formed protein to fold into a 3D shape. Different vaccine platforms including the full-length spike mRNA (Pfizer-BioNTech and Moderna vaccines), spike adenovirus-based DNA (AstraZeneca and Johnson & Johnson), and spike protein (Novavax) were developed to induce a specific neutralizing response against SARS-CoV-2. The content provided in this video is for informational and educational purposes only.\rPlease consult with a physician or healthcare professional regarding any medical or mental health related diagnosis or treatment. WebPfizer Vaccine Becomes DNA in Liver Cells. J. Med. Preclinical studies of COVID-19 mRNA vaccine BNT162b2, developed by Pfizer and BioNTech, showed reversible hepatic effects in animals that received the The pharmaceutical industry is dangerous to health. Read more about our fact-checking work here . Heres why: Lyons-Weiler J. Pathogenic priming likely contributes to serious and critical illness and mortality in COVID-19 via autoimmunity. You should not use it to replace any relationship with a physician or other qualified healthcare professional. J. Med. In this case, the author compared gene sequences to identify sections of human proteins that have portions similar to SARS-CoV-2 proteins and made a prediction that the similarities would cause immune responses against oneself. However, to someone trying to discern if the paper says what others are suggesting it says, a comparison of the methods and results sections with the subheadings used in the discussion section will quickly reveal that the topics addressed in the discussion go well beyond discussing protein sequences. Likewise, the Results section has five sentences. It is reverse transcribed by LINE-1 proteins of the cells. It is known from animal studies that it is also expressed early in embryonic development," explains Yang de Marinis. Pathogenic priming likely contributes to serious and critical illness and mortality in COVID-19 via autoimmunity. The whole process of Pfizers mRNA transfecting liver cells occurred within just six hours, per the study. Parents PACK. badbunny. WebThis and similar observations caused Spanish scientists to become curious about that actual contents of the vaccinations, since they wanted to understand what was giving rise to the magnetic effects. We have a new vaccine, and it needs to be tested in cell and animal models and also in humans, in various ways. However, the researchers at Lund University in Malm, Sweden, found that the mRNA vaccine enters human liver cells and triggers the cells DNA in the nucleus to increase the production of the LINE-1 gene expression to make mRNA. Professor at Infection Medicine YDM: About 18 percent of the vaccine accumulates in the liver just 30 minutes after the vaccine is injected in mice as reported by Pfizer in EMA assessment report, and therefore we chose to study liver cells. 5. Kyriakopoulos AM, Nigh G, McCullough PA, Seneff S. Cureus. In the study, the authors infected a human liver cell line with the Pfizer version of the COVID-19 vaccine. , conducted by researchers at Lund University in Sweden and published in a peer-reviewed journal, investigated the effect of Pfizers mRNA COVID-19 vaccine on a human cell line that was derived from liver cancer tissue. You should not consider the information in this site to be specific, professional medical advice for your personal health or for your family's personal health. For general inquiries, please use our contact form. And, again, while this journal allows for presenting a theory, the authors choice to use sections suggestive of a research study (methods, results, discussion) is somewhat misleading. Anti-vaccination and ivermectin proponents have gone bananas over the new study from the Lund University Fax: +46 (0)46 222 47 20, Invoice address: Box 188, 221 00 Lund YDM: The attention of the media and the general public reflects a concern among some regarding new vaccine technologies. The views expressed in this article are those of the author and do not reflect the official position of yourNEWS. Please select the most appropriate category to facilitate processing of your request. The posts share articles such as ones archived (here) and (here) saying the study, which was published in the Current Issues in Molecular Biology, shows mRNA from the Pfizer COVID-19 vaccine can enter human liver cells and convert to DNA as quickly as six hours after receiving a dose. The result might be surprising, but it is also a bit surprising that such studies do not seem to have been carried out before. The Swedish study also found spike proteins expressed on the surface of the liver cells that researchers say may be targeted by the immune system and possibly cause autoimmune hepatitis, as there [have] been case reports on individuals who developed autoimmune hepatitis after BNT162b2 vaccination.. For general feedback, use the public comments section below (please adhere to guidelines). The paper was published in April 2020, which was very early during the pandemic. The author described two potential mechanisms by which this could occur. "LINE-1 can convert RNA to DNA and has been shown to be found in tissues, including stem cells, in the human body. Microbiol Spectr. Your feedback is important to us. It has also been shown to affect immune responses. RNA is closely related to DNA, and one type, called messenger RNA or mRNA, sends instructions to the cell for different purposes. We show that the vaccine enters liver cells as Furthermore, a recent study showed that SARS-CoV-2 RNA can be reverse-transcribed and integrated into the genome of human cells. Two different antibody-dependent enhancement (ADE) risks for SARS-CoV-2 antibodies. Illustration, BNT162b2 mRNA levels in Huh7 cells treated with BNT162b2. 2021 Feb 24;12:640093. If you take just one thing away from this series, we hope you will have come to realize that science does not easily fit into a sound bite and, most often, it is not an either-or proposition, so if something sounds too simple, you probably need to ask more questions. Unable to load your collection due to an error, Unable to load your delegates due to an error. (In-vitro Swedish Study) Drbeen Medical Lectures 609K subscribers Join Subscribe 4.3M views Streamed 11 -. Webhttps://lnkd.in/dSz2cbQJ This was what everyone told me would not happen- that the RNA vaccines would not go into the DNA! MR: We understood that the study would attract attention, but we think it is self-evident that this type of research should be pursued. This also explains the choice of vaccine concentrations in our study, something we specifically address in the paper, which are 0.52% of the injection site concentration. The spike protein naturally occurs on the outside of the virus that causes Covid. Experts say that such interpretations mischaracterize the work and come to inaccurate conclusions. Data, especially data from vaccinated humans, will hopefully sort out the question marks. official website and that any information you provide is encrypted Immunohistochemistry using antibody binding to LINE-1 open reading frame-1 RNA-binding protein (ORFp1) on Huh7 cells treated with BNT162b2 indicated increased nucleus distribution of LINE-1. This genetic material contains instructions the body's cells use to build a protein found in a bacterium or virus, and that protein is then recognized by the immune system. The authors of thefirst reported caseof a healthy 35-year-old female who developed autoimmune hepatitis a week after her first dose of the Pfizer COVID-19 vaccine said that there is a possibility that spike-directed antibodies induced by vaccination may also trigger autoimmune conditions in predisposed individuals as it has been shown that severe cases of SARS-CoV-2 infection are characterized by an autoinflammatory dysregulation that contributes to tissue damage, which the viruss spike protein appears to be responsible for. BNT162b2 mRNA levels in Huh7 cells treated with BNT162b2. Your email address is used only to let the recipient know who sent the email. Pfizer Vaccine Becomes DNA in Liver Cells. Our Standards: The Thomson Reuters Trust Principles. Both boys were pronounced dead at home. MR: There is no reason for anyone to change their decision to take the vaccine based on this study. 2020;383:24392450. :\rBuy me a coffee :-) https://www.buymeacoffee.com/DrMobeenSyed\rBecome my patron: https://www.patreon.com/mobeensyed?fan_landing=true\rPayPal: https://paypal.me/mobeensyed?locale.x=en_US\r\r#drbeen #koolbeens #COVID\r\rChapters00:00 introduction00:45 Link to the summary01:00 Review of the reference material02:19 Cell preparation for the vaccine03:35 Vaccine addition to the cells06:00 RT-PCR for find RNA and then DNA13:39 Imaging of the cells and the LINE-1 protein, ORF-1 protein, and the DNA18:27 Measuring the volume of proteins in the cytosol and the nucleus22:35 DNA purification and verification28:41 Results28:42 LNP enters the liver cells efficiently29:33 LINE-1 gene is expressed30:46 ORF-1 found in cytosol and the nucleus36:01 DNA from the vaccine found in the cell43:30 Questions not answeredDisclaimer:\rThis video is not intended to provide assessment, diagnosis, treatment, or medical advice; it also does not constitute provision of healthcare services. doi: 10.1056/NEJMoa2027906. Tozinameran (BNT162b2) Vaccine: The Journey from Preclinical Research to Clinical Trials and Authorization. The experiment was conducted in a petri dish and aimed to answer the question of whether the mRNA in the vaccine could be converted to DNA. 19.9M. Still, the Swedish study only demonstrated that RNA had converted to DNA under the particular conditions created in the lab, not in a human body. Andy B. on LinkedIn: Pfizer Vaccine ADE is a condition in which the antibodies against a pathogen increase the ability of the pathogen to get into cells. 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No information in this video should ever be considered as a substitute for advice from a healthcare professional.\r\rURL list from Friday, Feb. 25 2022\r\rCIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML\rhttps://www.mdpi.com/1467-3045/44/3/73/htm\r\rFrontiers | Identification of Characteristic Genomic Markers in Human Hepatoma HuH-7 and Huh7.5.1-8 Cell Lines | Genetics\rhttps://www.frontiersin.org/articles/10.3389/fgene.2020.546106/full#:~:text=HuH%2D7%20(hereafter%20Huh7),experimental%20substitute%20for%20primary%20hepatocytes. Reuters has previously fact checked the claim that mRNA vaccines alter human DNA (here), (here), (here) and (here). By Meiling Lee. 1 . The researchers found that when the mRNA vaccine enters the human liver cells, it triggers the cells DNA, which is inside the nucleus, to increase the production of the LINE-1 gene expression to make mRNA. Bethesda, MD 20894, Web Policies Curr Issues Mol Biol. If you dont have time (or interest) in reviewing the primary source of the information, it is often best to let the information die in your feed or your inbox. Studies comparing the amount of live virus shed during infection with the amount of RNA detected by PCR have not shown the two measures to be well correlated. Turn on javascript for best performance. The results have in many cases been misinterpreted, the Q&A also stated. 2021;385:759760. The perfect family meal is here with healthy peas and beef full of protein! Below Australian experts address this concern Expert Comments: Professor Nigel McMillan Program Director of Infectious Diseases and Immunology at the Menzies Health Institute Queensland , Griffith Study: Pfizer Covid Vaccine Converts to DNA Inside Liver Cells. Table S1 summaries the vaccines Published on In the August 2022 James Gill paper titled Autopsy Histopathologic Cardiac Findings in 2 adolescents following the second COVID-19 vaccine dose, two teenage PCR primer set used to detect mRNA level and reverse-transcription of BNT162b2. Some of the vaccine dose is going to make it into the bloodstream, of course. Associate Professor MR: The study was performed on human liver cells from one cell linecell cultures used for research purposes. The study has received a lot of media attention, what are your thoughts on that? In a lab environment, i.e. [(accessed on 22 February 2022)]. The misconception related to this paper was that people thought it proved that ADE could occur after receipt of the COVID-19 vaccine. Invasion of Ukraine: Russia and Ukraine ready for second round of talks tonight. Humans have 20 commonly used amino acids and two that are used less frequently. Apart from any fair dealing for the purpose of private study or research, no YDM: One of the limitations of our study is that we don't know if what we observed in this cell line could also happen in cells of other tissue types, and this needs to be addressed in follow-up studies. Why liver cells and why the specific dose? Your browser has javascript turned off or blocked. sharing sensitive information, make sure youre on a federal Webpfizer vaccine become dna liver in cells 0 views Discover short videos related to pfizer vaccine become dna liver in cells on TikTok. DETAILS - Pfizer Vaccine Becomes DNA in The Human Liver Cells Huh7. WebSkip to topic navigation. In adifferent study, the spike proteins made in the body after receiving a Pfizer COVID-19 shot have been found on tiny membrane vesicles called exosomesthat mediate cell-to-cell communication by transferring genetic materials to other cellsfor at least four months after the second vaccine dose. The mRNA vaccines are entirely synthetic using lab synthesized RNA and lipid nanoparticles for delivery. We detected high levels of BNT162b2 in Huh7 cells and changes in gene expression of long interspersed nuclear element-1 (LINE-1), which is an endogenous reverse transcriptase. That is why it is impossible to alter your DNA with mRNA vaccines. In the cell line in a petri dish, you dont have those checks in place.. followers 129 videos. Accessibility statement. A cancer vaccine seemed a far-fetched idea just a few years ago, but now the shots exist and are gaining steam in the biotech community. The blood samples were analyzed for the virus that causes COVID-19 by subjecting them to polymerase chain reaction (PCR) testing. Pfizers covid jab contents enter the liver, alter human chromosomes and rewrites DNA. Click here:\rhttps://www.drbeen.com/yt-special-p/\r\rWant to support this work? They obtained more than 100 vials of the vaccine and tested them They found that 99% was Graphene Oxide (GO). part may be reproduced without the written permission. Some genetic mutations are inherited in the DNA that parents pass on to their children at birth, including gene changes that are known to increase the risk of developing cancer. (. Second, they measured LINE-1 activity. More than 215 million or 64.9 percent of Americans are fully vaccinated as of Feb. 28, with 94 million having received a booster dose. Chia PY, Ong SWX, Chiew CJ, et al. Huh7 cells were treated without (Ctrl) or with 0.5 g/mL of BNT162b2 for 6, 24, and 48 h. Genomic DNA was purified and digested with 100 g/mL RNase. At this stage, we do not know if DNA reverse transcribed from BNT162b2 is integrated into the cell genome. The messenger RNA (mRNA) fromPfizersCOVID-19vaccine is able to enter humanlivercells and is converted intoDNA, according to Swedish researchers at Lund University. MR: There is no reason for anyone to change their decision to take the vaccine based on this study. because Pfizer doesn't have any good studies on intra-cellular toxicity of their vaccine .. it leaves behind genetic markers that may indicate a change in DNA of the Two years later, we have ample evidence that previously infected or previously vaccinated people do not get more severely ill. A look at the authors affiliation and declaration of competing interest as well as an online search of his name and place of employment indicate that he probably has an anti-vaccine bias. YDM: A previous study from MIT has indicated that the SARS-CoV-2 virus mRNA can be converted to DNA and integrated into the human genome. Antibody levels were also measured in separate tests. -, Harris R.J., Hall J.A., Zaidi A., Andrews N.J., Dunbar J.K., Dabrera G. Effect of Vaccination on Household Transmission of SARS-CoV-2 in England. Moore also cautioned that the cells used in the study were quite different than primary cells in the body. The cell line came from liver cancer cells, which means their replication mechanisms were more active, and amplified the process in a way that would not have happened in healthier human cells, Moore said. They are not sure if it gets integrated in our DNA or not. The researchers write that the findings warrant a further Preclinical studies of COVID-19 mRNA vaccine BNT162b2, developed by Pfizer and BioNTech, showed reversible hepatic effects in animals that received the The Study Has Received A Lot of Media Attention, What Are Your Thoughts on That? A cancer vaccine seemed a far-fetched idea just a few years ago, but now the shots exist and are gaining steam in the biotech community. In the March 2022 issue of Parents PACK, we discussed some common misconceptions about science and scientists, and in the April 2022 issue, we discussed some surprising findings that were initially not believed but were ultimately proven to change our previously established understanding of science. Original video "Pfizer Vaccine Becomes DNA in Liver Cells. Pfizers COVID trials6 enrolled 46,331 participants in a Phase 3 clinical trial while Recombivax HB tested just 147 infants and children. 19.8M. Clipboard, Search History, and several other advanced features are temporarily unavailable. The study tested whether the vaccines A chocolaty goodness, an amazing comforting breakfast dish that the whole family will love! The content is provided for information purposes only. By using our site, you acknowledge that you have read and understand our Privacy Policy The authors likely opted to show the data this way because a lower cycle threshold means a sample had more virus, so when they plot the data, those higher on the graph represent samples with more virus compared with those lower on the graph. BNT: BNT162b2; L: DNA ladder; Ctrl1: cultured Huh7 cells; Ctrl2: Huh7 cells without BNT162b2 treatment collected at 6 h; Ctrl3: Huh7 cells without BNT162b2 treatment collected at 24 h; Ctrl4: Huh7 cells without BNT162b2 treatment collected at 48 h; Ctrl5: RNA from Huh7 cells treated with 0.5 g/mL of BNT162b2 for 6 h; Ctrl6: RNA from Huh7 cells treated with 0.5 g/mL of BNT162b2 for 6 h, digested with RNase. Case presentation: We report a case of hepatic artery occlusion after the first dose vaccination by ChAdOx1nCov-19. They certainly tell us something we did not know without the study, but it is important to understand the limitations of the data. -, Polack F.P., Thomas S.J., Kitchin N., Absalon J., Gurtman A., Lockhart S., Perez J.L., Perez Marc G., Moreira E.D., Zerbini C., et al. According to the study, once the mRNA vaccine enters the human liver cells, it triggers the cell's DNA inside the nucleus to produce more of the LINE-1 gene expression to make mRNA. Huh7 cells were treated, Immunohistochemistry of Huh7 cells treated, Immunohistochemistry of Huh7 cells treated with BNT162b2 on LINE-1 protein distribution. Mandatory vaccinations, the segregation of citizens, and the promotion of inequality in the modern democracy of Greece and other democratic countries in the era of COVID-19. We show that the vaccine enters liver cells as early as six hours after the vaccine has been administered. Finally, as the authors pointed out, expression of LINE-1 has been shown to increase during viral infections, including with SARS-CoV-2 virus. These tools for evaluating information may be useful: Categories: As such, by definition, a modeling study cannot serve as proof. To understand why this is unlikely, one needs to know a bit about protein chemistry: An analysis like the one in this paper may allow one to create an interesting hypothesis, but it does not provide evidence of a concern. WebIn total, 300 liver transplant recipients and 122 healthcare workers (HCW) were enrolled in this study. Please enable it to take advantage of the complete set of features! Giving to Lund The more virus in a sample, the fewer cycles it would take to detect RNA. This is currently how depression and, Small passerine birds, such as blue and great tits, avoid breeding in urban areas where there are many non-native trees. 2022 Dec 9;14(12):e32361. This is part of APs effort to address widely shared misinformation, including work with outside companies and organizations to add factual context to misleading content that is circulating online. 5 Notably, mutations in the BRCA tumor-suppressor genes can be inherited. helps train the body to recognize a protein from the coronavirus to trigger an immune response. This will lead to some parts of our website to not work properly or at all. time: 15 Cooks in: 1:40 Ready in: 1:55 Ingredients 1 kg beef flatiron 4 tbsp extra-virgin olive oil 1 garlic clove, finely chopped 1 tbsp tomato paste cup []. 2022 Feb 28;194(8):E297-E305. They are not sure if it gets integrated in our DNA or not. Brighter days are ahead and a vaccine is coming! 2023 Jan 19. Two of the authors, Associate Professor Yang de Marinis (YDM) and Professor Magnus Rasmussen (MR), share their views. Each dot on the table represents a study subject. Pfizer didnt comment on the findings of the Swedish study and said only that its mRNA vaccine does not alter the human genome. Lund University 3401 Civic Center Blvd. As we discussed in, The quantity of a genetic tool called LINE-1, The presence of DNA sequences similar to the vaccine RNA in the nuclei of these cells, Perhaps most importantly, this experiment was done on cells being grown in a lab. We have a new vaccine, and it needs to be tested in cell and animal models and also in humans, in various ways. Magnus Rasmussen WebImmunogenicity after SARS-CoV-2 vaccination is known to be impaired in liver transplant (LT) recipients, but the results after the application of a third dose show significant improvement in seroconversion rates. The results have been widely discussed across social media but the results have in many cases been misinterpreted. -, Walsh E.E., Frenck R.W., Jr., Falsey A.R., Kitchin N., Absalon J., Gurtman A., Lockhart S., Neuzil K., Mulligan M.J., Bailey R., et al. Reverse transcription is when DNA is made from RNA, whereas the normal transcription process involves a portion of the DNA serving as a template to make an mRNA molecule inside the nucleus. in petri dishes, the researchers added Pfizer BioNTech's vaccine to a cell line that originally came from a human liver tumour. Yau K, Chan CT, Abe KT, Jiang Y, Atiquzzaman M, Mullin SI, Shadowitz E, Liu L, Kostadinovic E, Sukovic T, Gonzalez A, McGrath-Chong ME, Oliver MJ, Perl J, Leis JA, Bolotin S, Tran V, Levin A, Blake PG, Colwill K, Gingras AC, Hladunewich MA. (AP Photo/Jae C. Hong, File), Connect with the definitive source for global and local news, A clip being shared online in recent days shows three doctors, who have spread misinformation about the vaccines in the past, discussing the Swedish study and falsely claiming it demonstrates that the Pfizer vaccine reverse transcribes and installs DNA into the human genome, leading to harmful effects. This was what everyone told me would not go into the cell with. After receipt of the cells of talks tonight lot of media attention, what are thoughts! Our DNA or not: \rhttps: //www.drbeen.com/yt-special-p/\r\rWant to support this work 14 ( ). Your collection due to an error, unable to load your delegates due to an.. Than 100 vials of the Swedish study ) Drbeen Medical Lectures 609K Join... Associate Professor Yang de Marinis ( YDM ) and Professor Magnus Rasmussen ( mr ) share. S. Cureus hours, per the study, but it is impossible to alter your DNA with mRNA are! 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